日粮维E抑制空肠上皮细胞增殖，进而影响肠道形态、消化酶活性和养分转运因子

Cancan Chen Zhaobin Wang Jianzhong Li Yali Li Pengfei Huang Xueqin Ding Jia Yin Shanping He Huansheng Yang Yulong Yin

维E是仔猪日粮配方中不可缺少的维生素之一。除此之外，它影响着小肠组织，尤其是肠道上皮细胞的功能。前人的研究很少关注维E对肠道组织影响(尤其是消化吸收方面)。已经证实维E可以抑制一些细胞的分化。相关阅读：仔猪肠道健康的福音--斯福

本试验在于研究维E是否通过影响肠道细胞的分化进而影响肠道功能。试验选用30头21日龄断奶仔猪(约克夏×杜洛克)，初始重6.36±0.55kg，随机分为5个处理组。分别为：对照组(0 IU)、16 IU、32 IU、80 IU、160 IU。试验期14天。试验结束后，屠宰试验动物，收集血液和组织样品。

(图：维E)

试验结果表明维E不影响动物生长性能。维E可以提高空肠隐窝深度(线性，P=0.056)和绒毛宽度(线性，P<0.05)。与对照组相比，80IU维E组蔗糖酶活性显著降低(P<0.05)。并且空肠隐窝深度和细胞分化也有显著降低(P<0.05)。

试验说明日粮维E可能抑制断奶仔猪空肠上皮细胞增殖，进而影响肠道形态和功能。

Dietary vitamin E affects small intestinal histomorphology, digestive enzyme activity, and the expression of nutrient transporters by inhibiting proliferation of intestinal epithelial cells within jejunum in weaned piglets

Cancan Chen Zhaobin Wang Jianzhong Li Yali Li Pengfei Huang Xueqin Ding Jia Yin Shanping He Huansheng Yang Yulong Yin

Vitamin E (VE) is an indispensable vitamin in piglet feed formula. Among other things, it affects tissues including small intestine tissues and in particular its major unit intestinal epithelial cells. Previously, limited in vivo experiments have focused on the effect of VE on the intestine, particularly digestion and absorption. VE has been shown to inhibit proliferation of some types of cells. This experiment was conducted to test the hypothesis that VE affects intestinal functions by influencing the intestinal epithelial cell proliferation. Thirty 21-d old weaned [(Yorkshire × Landrace) × Duroc] piglets with BWs of 6.36 ± 0.55 kg were randomly divided into five VE-containing feeding formula groups. The treatments were (i) 0 IU (control), (ii) 16 IU, (iii) 32 IU, (iv) 4. 80 IU, and (v) 5. 160 IU. The treatments lasted 14 d. At the end of the experiment, all subjects were sacrificed to obtain blood and tissue samples. The results suggest that VE did not affect the growth performance. VE did tend to decrease jejunal crypt depth (linear, P = 0.056) and villus width (linear, P < 0.05). Sucrase activity significantly decreased in the adding 80 IU VE compared with the control (P < 0.05). Jejunal crypt, cell proliferation in 80 IU group significantly decreased compared with the control group (P < 0.05). This study suggests that dietary VE may affect intestinal morphology and functions by inhibiting weaned piglet jejunal epithelial cell proliferation.